The Emirates Racing Associates upheld the disqualification of Brass Hat from the March 25 Dubai World Cup on Tuesday after hearing an appeal by Brass Hat's owner and trainer.

The disqualification will cost Brass Hat's owner, Fred Bradley, the $1.2 million second-place share of the World Cup's $6 million purse. Stewards at Nad al Sheba Racecourse, where the World Cup was run, disqualified Brass Hat after he tested positive for a banned corticosteroid following the race.

Brass Hat's trainer, William "Buff" Bradley - the son of Fred Bradley - argued in his appeal that he administered methyl prednisolone acetate, a potent anti-inflammatory drug, 28 days before the race, five days outside of a guideline contained in a document distributed by a representative of the World Cup. Buff Bradley said on Tuesday that he never would have had his veterinarian inject the drug if it was not listed on the document.

"We're really disappointed, obviously, and I think that anyone who is considering going over there to race needs to take a real close look at everything," Buff Bradley said on Tuesday after being informed of the ruling.

Bradley said that the Emirates Racing Associates argued during the Monday hearing that the document was not endorsed by the association, as Bradley believed. Emirates Racing Associates conducts racing at Nad al Sheba under a zero-tolerance drug policy. The document cited by Bradley's legal team states that "in Dubai, there are no tolerance levels, only negative or positive," in reference to test results.

Slide show   Week in Sports Pictures Pain on the skating rink, flying high on the hardwood, upsets on the football field, and more. more photos

The appeals hearing was conducted via conference calls between Brass Hat's legal team in Frankfort, Ky., and the Emirates officials in Dubai. The hearing lasted two hours Monday, and Emirates Racing Associates handed down the ruling Tuesday morning.

Buff Bradley said that the officials from Dubai said that no further appeals of the ruling could be considered.

MORE FROM HORSE RACING

Churchill Downs doubles its night racing

Churchill Downs doubles its night racing Hall of Fame trainer Bobby Frankel dies at 68 Perfect Zenyatta (14-0) awaits breeding career Racing's Year of the Girl Zenyatta beats the boys in Breeders’ Cup Classic   Video: Filly Rachel Alexandra wins Preakness thriller   Video: Watch replay of Mine That Bird's victory Interactive: Triple Crown winners, near misses Vote: When will we see next Triple Crown winner? Equibase: Quick results from top tracks   Add Horse racing headlines to your news reader: